Histone H3 [Dimethyl Lys4] Antibody

• Supplier: Novus Biologicals
• Catalog: NB21-1022
• Prices: $199.00, $349.00
• Sizes: 25 µg, 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB ChIP DB ICC/IF
• Species Reactivities: Human, C. elegans, Rat, Chimpanzee, Plant
• Antigen: Synthetic dimethylated peptide surrounding Lysine 4 of human Histone H3.2 [Swiss Prot Q71DI3].
• Purity/Format: Immunogen affinity purified
• Blocking Peptide: Histone H3 [Dimethyl Lys4] Blocking Peptide
• Description: Rabbit Polyclonal
• Gene: HIST2H3C
• Conjugate: Unconjugated

Product images

Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - H3K4Me2 immunoblot in Jurkat cells. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - Histone H3 was tested at 1:100 in Hela cells against Dylight 488 (Green). Nuclei were illuminated blue with DAPI.

Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1022 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.

Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - Western blot analysis of H3 K4me2 in HeLa histone preps at 1:1000.

Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - WB analysis of H3 K4me2 in C. elegans embryo lysate.

Dot Blot: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - Dot blot analysis of H3 K4me2 using the peptides stated above in 25, 10, 3 and 1 picomoles of peptide.

Histone H3 [Dimethyl Lys4] Antibody [NB21-1022]