Histone H4 [Monomethyl Lys20] Antibody

• Supplier: Novus Biologicals
• Catalog: NB21-2088
• Prices: $199.00, $349.00
• Sizes: 25 µg, 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB ChIP DB ICC/IF
• Species Reactivities: Human, C. elegans, Rat, Chimpanzee, Plant
• Antigen: Synthetic monomethylated peptide surrounding Lysine 20 of human Histone H4 [Swiss Prot P62805].
• Purity/Format: Immunogen affinity purified
• Blocking Peptide: Histone H4 [Monomethyl Lys20] Blocking Peptide
• Description: Rabbit Polyclonal
• Gene: HIST1H4I
• Conjugate: Unconjugated

Product images

Western Blot: Histone H4 [Monomethyl Lys20] Antibody [NB21-2088] - Western blot analysis of HeLa histone prep lysate (A) and NIH 3T3 histone prep lysate (B) using Histone H4 [Monomethyl Lys20] antibody at 2 ug/ml.

Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody [NB21-2088] - Histone H4 K20me1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Chromatin Immunoprecipitation: Histone H4 [Monomethyl Lys20] Antibody [NB21-2088] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-2088 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.

Western Blot: Histone H4 [Monomethyl Lys20] Antibody [NB21-2088] - WB detection of H4K20me1 in NIH/3T3 histone preps.

Western Blot: Histone H4 [Monomethyl Lys20] Antibody [NB21-2088] - WB analysis of Histone H4K20me1 in C. elegans embryo lysate.

Dot Blot: Histone H4 [Monomethyl Lys20] Antibody [NB21-2088] - Dot blot analysis of H4K20me1 using peptides stated above in picomoles of peptide.

Histone H4 [Monomethyl Lys20] Antibody [NB21-2088]