Western blot shows lysates of NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Goat Anti-RatIL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Recombinant Rat IL‑6 (Catalog # 506-RL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant RatIL‑6 (0.6 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506). The ND50 is typically 0.1-0.5 µg/mL.
IL‑6 was detected in immersion fixed activated mouse T Cells using 15 µg/mL Rat IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.