Western blot shows lysates of mouse liver tissue and bEnd.3 mouse brain endothelial cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2125) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for LYVE‑1 at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LYVE‑1 was detected in perfusion fixed frozen sections of mouse liver using 15 µg/mL Mouse LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2125) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of endothelial cells in sinusoids. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.