Histone H3 [Monomethyl Lys9] Antibody

• Supplier: Novus Biologicals
• Catalog: NB21-1071
• Prices: $199.00, $349.00
• Sizes: 25 µg, 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB ChIP DB ICC/IF
• Species Reactivities: Human, C. elegans, Rat, Chimpanzee, Plant
• Antigen: Synthetic monomethylated peptide surrounding Lysine 9 of human Histone H3.2 [Swiss Prot Q71DI3].
• Purity/Format: Immunogen affinity purified
• Blocking Peptide: Histone H3 [Monomethyl Lys9] Blocking Peptide
• Description: Rabbit Polyclonal
• Gene: HIST2H3C
• Conjugate: Unconjugated

Product images

Western Blot: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - WB analysis of H3K9me1 in C. elegans embryo lysate.

Immunocytochemistry: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - Immunofluorescence: [NB21-1071] - Histone H3.2 K9me1 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).

Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1071 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.

Western Blot: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - WB analysis of H3K9me1 in NIH/3T3 histone preps.

Dot Blot: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - Dot blot analysis of H3K9me1 in picomoles of peptide.

Histone H3 [Monomethyl Lys9] Antibody [NB21-1071]