Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - WB analysis of H3K9me2 in C. elegans embryo lysate.
Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Histone H3.2 K9me2 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).
Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1072 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Western blot analysis of Histone H3K9me2 on HeLa histone preps.
Dot Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Dot blot analysis of H3K9me2 in picomoles of peptide.
Histone H3 [Dimethyl Lys9] Antibody [NB21-1072]