Histone H3 [p Ser28, Trimethyl Lys27] Antibody

• Supplier: Novus Biologicals
• Catalog: NB21-1193
• Prices: $349.00
• Sizes: 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB ChIP DB ICC/IF
• Species Reactivities: Human, C. elegans, Rat, Chimpanzee, Plant
• Antigen: Synthetic trimethylated/phosphorylated peptides surrounding lysine 27 and serine 28 of human Histone H3 [Swiss Prot Q71DI3].
• Purity/Format: Immunogen affinity purified
• Description: Rabbit Polyclonal
• Gene: HIST2H3C
• Conjugate: Unconjugated

Product images

Western Blot: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - H3 K27Me3 antibody was tested in 1) HeLa histone prep 2) NIH-3T3 histone prep and 3) C. elegans embryo cell lysate.

Immunocytochemistry: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - Immunofluorescence: [NB21-1193] - Histone H3 K27me3/pS28 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Chromatin Immunoprecipitation: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-1193 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.

Immunocytochemistry: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - Immunofluorescence: [NB21-1193] - Histone H3 K27me3/pS28 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193]

Dot Blot: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - Dot Blot analysis of NB21-1193 using the peptides listed above in 100, 10, and 1 picomoles of peptide.