Histone H3 [p Thr3, Dimethyl Lys4] Antibody

• Supplier: Novus Biologicals
• Catalog: NB21-1013
• Prices: $199.00, $349.00
• Sizes: 25 µg, 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB ChIP DB ICC/IF
• Species Reactivities: Human, C. elegans, Rat, Chimpanzee, Plant
• Antigen: Synthetic dimethylated/phosphorylated peptides surrounding Lysine 4 and Threonine 3 of human Histone H3 [Swiss Prot Q71DI3].
• Purity/Format: Immunogen affinity purified
• Description: Rabbit Polyclonal
• Gene: HIST2H3C
• Conjugate: Unconjugated

Product images

Western Blot: Histone H3 [p Thr3, Dimethyl Lys4] Antibody [NB21-1013] - WB analysis of Histone H3 pT3/K4me2 in NIH-3T3 histone prep lysate.

Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr3, Dimethyl Lys4] Antibody [NB21-1013] - Histone H3 pT3/K4me2 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Chromatin Immunoprecipitation: Histone H3 [p Thr3, Dimethyl Lys4] Antibody [NB21-1013] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1013 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.

Histone H3 [p Thr3, Dimethyl Lys4] Antibody [NB21-1013]

Dot Blot: Histone H3 [p Thr3, Dimethyl Lys4] Antibody [NB21-1013] - Dot Blot analysis of H3pT3/K4me2 using the peptides listed above in 100, 10 & 1 picomoles of peptide.