Histone H3 [Dimethyl Lys18] Antibody

• Supplier: Novus Biologicals
• Catalog: NB21-1142
• Prices: $199.00, $349.00
• Sizes: 25 µg, 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB ChIP DB ICC/IF
• Species Reactivities: Human, C. elegans, Rat, Chimpanzee, Plant
• Antigen: Synthetic dimethylated peptide surrounding Lysine 18 of human Histone H3.2 [Swiss Prot Q71DI3].
• Purity/Format: Immunogen affinity purified
• Blocking Peptide: Histone H3 [Dimethyl Lys18] Blocking Peptide
• Description: Rabbit Polyclonal
• Gene: HIST2H3C
• Conjugate: Unconjugated

Product images

Western Blot: Histone H3 [Dimethyl Lys18] Antibody [NB21-1142] - WB analysis of H3.2 K18me2 in C. elegans embryo lysate.

Immunocytochemistry: Histone H3 [Dimethyl Lys18] Antibody [NB21-1142] - Immunofluorescence: [NB21-1142] - Histone H3.2 K18me2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).

Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys18] Antibody [NB21-1142] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1142 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.

Western Blot: Histone H3 [Dimethyl Lys18] Antibody [NB21-1142] - WB detection of Histone H3 [dimethyl Lys18] in NIH 3T3 histone preps.

Dot Blot: Histone H3 [Dimethyl Lys18] Antibody [NB21-1142] - Dot blot analysis of H3K18me2 antibody using peptides stated above in 1.0, 0.3 and 0.1 picomoles.

Histone H3 [Dimethyl Lys18] Antibody [NB21-1142]