Western Blot: Nup53 Antibody [NB100-93322] - Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP35 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry/Immunofluorescence: Nup53 Antibody [NB100-93322] - Showing nuclear co-localization with p300 in PANC-1 cells using anti-Nup53 antibody (green). Image from verified customer review.
Immunohistochemistry: Nup53 Antibody [NB100-93322] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- NUP35 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: Nup53 Antibody [NB100-93322] - FFPE section of human prostate carcinoma. Antibody: affinity purified rabbit anti-NUP35 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB