Western Blot: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] Antibody [NB21-1202] - WB analysis of Histone H3 R2me2s/K4Me2 in C. elegans embryo lysate.
Immunocytochemistry: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Immunofluorescence: Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] Antibody [NB21-1202] - Histone H3.2 R2me2s/K4me2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 594 (red).
Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] Antibody [NB21-1202] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1202 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
Western Blot: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] Antibody [NB21-1202] - WB analysis of Histone R2 in NIH 3T3 histone preps.
Dot Blot: Histone H3 [Dimethyl Lys4, Sym-dimethyl Arg2] Antibody [NB21-1202] - Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] Antibody [NB21-1202] - Dot blot analysis of H3R2me2s/K4Me2 in picomoles of peptide. Lane 1: R2Me2 only, Lane 2: Both Modifications, Lane 3: K4Me2