Western blot analysis of extracts from ZR-75-1 and HeLa cells using 4F2hc/CD98 (D6O3P) Rabbit mAb.
Immunoprecipitation of 4F2hc/CD98 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or 4F2hc/CD98 (D6O3P) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using 4F2hc/CD98 (D6O3P) Rabbit mAb.
Confocal immunofluorescent analysis of HeLa (left) and SW620 (right) cells using 4F2hc/CD98 (D6O3P) Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of HeLa cells using 4F2hc/CD98 (D6O3P) Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 conjugate) #4412 was used as a secondary antibody.