Histone H4 [ac Lys8] Antibody

• Supplier: Novus Biologicals
• Catalog: NB21-2044
• Prices: $199.00, $349.00
• Sizes: 25 µg, 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB ChIP DB ICC/IF
• Species Reactivities: Human, C. elegans
• Antigen: Synthetic acetylated peptide surrounding lysine 8 of human histone H4 [Swiss Prot P62805].
• Purity/Format: Immunogen affinity purified
• Description: Rabbit Polyclonal
• Gene: HIST1H4I
• Conjugate: Unconjugated

Product images

Western Blot: Histone H4 [ac Lys8] Antibody [NB21-2044] - WB analysis of H4 K8ac in 1) HeLa histone prep 2) 3T3 histone prep and 3) C. elegans embryo lysate.

Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody [NB21-2044] - H4 K8ac antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NB21-2044] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-2044 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.

Dot Blot: Histone H4 [ac Lys8] Antibody [NB21-2044] - Dot Blot analysis of H4 K8ac using the peptides listed above in 100, 10, and 1 picomoles of peptide.

Histone H4 [ac Lys8] Antibody [NB21-2044]