Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb (ChIP formulated)
| Name | Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb (ChIP formulated) |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 11971 |
| Prices | $287.00 |
| Sizes | 100 µl (10 immunoprecipitations) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | D5B10 |
| Applications | ChIP |
| Species Reactivities | Human, Mouse, Rat, Monkey |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser463/465 of human Smad1 and Smad5 protein. |
| Description | Rabbit Monoclonal |
| Gene | SMAD1 |
| Conjugate | ChIP formulated |
| Supplier Page | Shop |
Product images
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 MCF7 cells treated with Human BMP2 #4697 (50 ng/ml) for 1 hr and either 5 μl of Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb (ChIP formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human ID1 Promoter Primers #5139, human SMAD6 promoter primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.