Smad1 Antibody
| Name | Smad1 Antibody |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 9743 |
| Prices | $246.00 |
| Sizes | 100 µl (10 western blots) |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | WB IP ChIP |
| Species Reactivities | Human, Mouse, Monkey |
| Antigen | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser190 of human Smad1 |
| Description | Rabbit Polyclonal |
| Gene | SMAD1 |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from various cell lines using Smad1 Antibody.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 MCF7 cells treated with Human BMP2 #4697 (50 ng/ml) for one hour and either 20 μl of Smad1 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human ID1 Promoter Primers #5139, human SMAD6 promoter primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.