Western blot shows lysates of human brain cortex and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRRTM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
LRRTM2 was detected in immersion fixed frozen sections of mouse neural tube (E9.5) using Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
LRRTM2 was detected in immersion fixed frozen sections of embryonic mouse neural tube (E10.5) using 10 µg/mL Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
LRRTM2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.