Recombinant Canine IL‑6 (Catalog # 1609-CL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑6 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1609). The ND50 is typically 0.4-2.0 µg/mL.
IL‑6 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) stimulated with PHA using Goat Anti-Canine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1609) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CLL‑1390 canine leukocytic round cell neoplasia cell line was stained with Goat Anti-Canine IL‑6 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF1609, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.