Western blot shows lysates of human stomach tissue and human small intestine tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human GFR alpha ‑3/GDNF R alpha ‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF670) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for GFR alpha ‑3/GDNF R alpha ‑3 at approximately 55 and 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.