SP1 Antibody

• Supplier: Novus Biologicals
• Catalog: NB600-232
• Prices: $359.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB ChIP ICC/IF IHC IHC-P IP
• Species Reactivities: Human, Rat, Bovine, Goat, Monkey
• Antigen: The immunogen recognized by this antibody maps to a region between residues 700 and 740 using the numbering given in entry XP_028606.4 (GeneID 6667).
• Purity/Format: Immunogen affinity purified
• Description: Rabbit Polyclonal
• Gene: DAND5
• Conjugate: Unconjugated

Product images

Immunohistochemistry-Paraffin: SP1 Antibody [NB600-232] - Sample: FFPE sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-Sp1 used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB

Western Blot: SP1 Antibody [NB600-232] - Detection of Human SP1 on HeLa whole cell lysate using NB600-232. Subsequently, NB600-232 was used at 0.04 mcg/ml for WB.

Western Blot: SP1 Antibody [NB600-232] - Samples : A. Whole cell lysate (50 ug for E; 10 ug for T) from HEK 293T cells that were mock transfected (E) or transfected with an Sp1 expression construct (T). B. Whole cell lysate (~700 ug) from HEK 293T cells. Antibody : A. Affinity purified rabbit anti-Sp1 antibody used at the indicated concentrations. B. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibodies BL937, using each antibody at 3.75 ug/mg lysate. Subsequently, was used at 0.04 ug/ml for WB. Detection : Chemiluminescence with an exposure time of 10 seconds (A) or 1 min (B).