Western Blot: IKK beta Antibody [NB600-477] - Western blot of anti-IKKBeta antibody reacted with HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:500 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1h. Washes with TBS preceded color development.
Immunohistochemistry-Paraffin: IKK beta Antibody [NB600-477] - Paraffin-embedded mouse brain sections (IKK beta KO or IKK beta WT) were stained with IKK beta (green). Note: during staining, remember to add 0.1-0.3% Triton-100 in the antibody incubation buffer. See PMID 25253847 for further details regarding the experiemental conditions. Image from verified customer review.
Immunohistochemistry-Paraffin: IKK beta Antibody [NB600-477] - Human placenta tissue.