Western blot shows 25 ng of Recombinant Human TIM‑4 His-tag (Catalog # 2929-TM), Recombinant Human TIM‑1/KIM‑1/HAVCR (Catalog # 1750-TM), Recombinant Human TIM‑3 Fc Chimera (Catalog # 2365-TM), Recombinant Mouse TIM‑1/KIM‑1/HAVCR (Catalog # 1817-TM), Recombinant Mouse TIM‑2 (Catalog # 1885-TM), Recombinant Mouse TIM‑3 Fc Chimera (Catalog # 1529-TM), Recombinant Mouse TIM‑4 (Catalog # 2826-TI), Recombinant Mouse TIM-5, and Recombinant Mouse TIM-6. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human TIM‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2929) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TIM‑4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.