Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL) stimulates proliferation in BaF3 mouse pro‑B cell line transfected with mouse Flt‑3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Flt‑3 Ligand (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Flt‑3 Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF427). The ND50 is typically 0.05‑0.25 µg/mL.
Flt‑3 Ligand was detected in immersion fixed HT‑2 mouse T cell line using Goat Anti-Mouse Flt‑3 Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF427) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.