Smad4 Antibody
| Name | Smad4 Antibody |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 9515 |
| Prices | $129.00, $239.00 |
| Sizes | 40 µl (4 western blots), 100 µl (10 western blots) |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | WB ChIP |
| Species Reactivities | Human, Mouse, Rat, Monkey |
| Antigen | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the residues surrounding Pro278 of human Smad4 |
| Description | Rabbit Polyclonal |
| Gene | SMAD4 |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from COS, NIH3T3, PC12, and SK-N-MC cells, using Smad4 Antibody.
Western blot analysis of extracts from NIH/3T3 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Smad4 siRNA I (Mouse Specific) (+), using Smad4 Antibody #9515 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The Smad4 Antibody confirms silencing of Smad4 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HaCaT cells treated with Human TGF-β3 #3706 (7ng/ml) for 1 h and either 20 μl of Smad4 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CDKN1A Intron 1 Primers #4669, SimpleChIP® Human ID1 Promoter Primers #5139, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Product References
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Upton PD, Davies RJ, Trembath RC, Morrell NW. J Biol Chem. 2009 Jun 5;284(23):15794-804.
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Ding Y, Kim JK, Kim SI, Na HJ, Jun SY, Lee SJ, Choi ME. J Biol Chem. 2010 Nov 26;285(48):37909-19.
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Long X, Miano JM. J Biol Chem. 2011 Aug 26;286(34):30119-29.
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Wang J, Wang Y, Wang Y, Ma Y, Lan Y, Yang X. J Biol Chem. 2013 Apr 12;288(15):10418-26.
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