Western Blot: OXTR Antibody [NBP1-28487] - (0.5ug/ml) staining of Rat Uterus (U) and Rat Skeletal Muscle (M) lysates (35ug protein in RIPA buffer). Data obtained from Maree Gould, University of Otago, New Zealand. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: OXTR Antibody [NBP1-28487] - Staining of Human Prostate stromal cells. Overnight incubation at 4C and detected with Alexafluor secondary conjugated antibody.
Immunohistochemistry-Paraffin: OXTR Antibody [NBP1-28487] - (2ug/ml) staining of paraffin embedded Human Prostate Cancer. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Western Blot: OXTR Antibody [NBP1-28487] - Staining of lysates of PC3 cells treated in several ways (50,000 cells per lane). Reprobed for alpha-tubulin (50kDa) to serve as loading control (OXTR antibody incubation was overnight. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: OXTR Antibody [NBP1-28487] - (5ug/ml) staining of PC3 cells. Detected by DAB. This data was obtained using a previous batch