Western Blot: p27/Kip1 Antibody [NBP1-32213] - Whole cell extracts (30 ug) was separated by 12 % SDS-PAGE, and blotted with p27 Kip1 antibody diluted by 1:500
Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody [NBP1-32213] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p27 Kip1 protein stained by p27 Kip1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.
Immunohistochemistry-Frozen: p27/Kip1 Antibody [NBP1-32213] - Frozen sectioned adult mouse retina. Green: p27 Kip1 protein stained by p27 Kip1 antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI.
Western Blot: p27/Kip1 Antibody [NBP1-32213] - Sample (50 ug of whole cell lysate) A: CCRF-SB 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: p27/Kip1 Antibody [NBP1-32213] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody [NBP1-32213] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Frozen: p27/Kip1 Antibody [NBP1-32213] - Staining of human ulcerative colitis tissue using anti-p27 Kip1 antibody.
Immunoprecipitation: p27/Kip1 Antibody [NBP1-32213] - Immunoprecipitation of p27 Kip1 protein from MCF-7 whole cell extracts using 5 ug of p27 Kip1 antibody. Western blot analysis was performed using p27 Kip1 antibody.EasyBlot anti-Rabbit IgG was used as a secondary reagent.