Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody [NB110-58783] - Detection of Spectrin Beta-III in human hepatocytes using NB110-58783.
Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody [NB110-58783] - Spectrin beta 3 antibody was tested in Neuro 2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunoprecipitation: Spectrin beta 3 Antibody [NB110-58783] - Detection of Human SPTBN2/SCA5 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SPTBN2/SCA5 antibody NB110-58783 used for IP at 3 mcg/mg lysate. SPTBN2/SCA5 was also immunoprecipitated by rabbit anti-SPTBN2/SCA5 antibody NB110-58346, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.