Western blot shows lysates of human heart tissue, human skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue, and mouse heart tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human Caveolin-3 Monoclonal Antibody (Catalog # MAB6706) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caveolin-3 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Caveolin-3 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human Caveolin-3 Monoclonal Antibody (Catalog # MAB6706) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to sarcolemma of muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.