Western blot shows lysates of human liver tissue and human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PRELP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6447) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PRELP at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Recombinant Human PRELP (Catalog # 6447-PR) inhibits Recombinant Mouse TRANCE induced osteoclast-like cell formation in the RAW 264.7 mouse monocyte/macrophage cell line in a dose-dependent manner (orange line), as measured by the TRAP (tartrate‑resistant acid phosphatase) solution assay. Inhibition of Recombinant Mouse TRANCE (5 ng/mL) activity elicited by Recombinant Human PRELP (5 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human PRELP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6447). The ND50 is typically 0.2-1.2 µg/mL in the presence of Recombinant Mouse M‑CSF (20 ng/mL).
PRELP was detected in immersion fixed human mesenchymal stem cells differentiated into chondrocytes using Sheep Anti-Human PRELP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6447) at 10 µg/mL overnight at 4 °C. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.