Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse YM1/Chitinase 3‑like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2446) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for YM1/Chitinase 3‑like 3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of mouse bone marrow, loaded at 0.2 mg/mL. A specific band was detected for YM1/Chitinase 3‑like 3 at approximately 49 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse YM1/Chitinase 3‑like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2446) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.