Western blot shows lysates of ST‑2 mouse bone marrow-derived stromal cell line and TT human medullary thyroid cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse Polypeptide GalNac Transferase 10/GALNT10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7575) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Polypeptide GalNac Transferase 10/GALNT10 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Polypeptide GalNac Transferase 10/GALNT10 was detected in perfusion fixed frozen sections of mouse testis using Sheep Anti-Human/Mouse Polypeptide GalNac Transferase 10/GALNT10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7575) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Golgi organelles in spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.