Western Blot: TRIM23 Antibody [NB100-78620] - Sample(30 ug of whole cell lysate)A:HeLa S3(GTX14654)10% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: TRIM23 Antibody [NB100-78620] - HCT116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRIM23 protein stained by TRIM23 antibody [N1], N-term diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: TRIM23 Antibody [NB100-78620] - Paraffin-embedded gastric CA, using antibody at 1:100 dilution.
Immunocytochemistry/Immunofluorescence: TRIM23 Antibody [NB100-78620] - Paraformaldehyde-fixed HeLa, using TRIM23 antibody (Green) at 1:500 dilution.
Immunocytochemistry/Immunofluorescence: TRIM23 Antibody [NB100-78620] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TRIM23 antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with red at 1:2500.