Western Blot: Tubulin [ac Lys40] Antibody (6-11B-1) [NB600-567] - WB validation of monoclonal anti-Acetylated Tubulin antibody clone: 6-11B-1 at 1:2000 dilution with goat anti-Mouse IgG-Peroxidase secondary antibody - chemiluminescent substrate development. The lysates employed were from cell lines: 1. HeLa, 2. U87, 3. COS7, 4. P19, 5. RAT2, 6. CHO, 7. MDBK and 8. MDCK
Immunocytochemistry/Immunofluorescence: Tubulin [ac Lys40] Antibody (6-11B-1) [NB600-567] - HeLa cells were fixed with 4% paraformaldehyde followed permeabilization with 0.5% Triton X-100. Fixed cells were stained with 1:2,000 monoclonal Tubulin, Acetylated antibody Clone: 6-11B-1. The antibody was detected using goat anti-Mouse IgG, Cy3 conjugate followed by counterstaining with DAPI (blue).
Immunohistochemistry: Tubulin [ac Lys40] Antibody (6-11B-1) [NB600-567] - Nerve pathways in the embryonic Zebrafish brain and spinal cord revealed by Monoclonal Anti-Acetylated Tubulin (cat#NB600-567). Immunoperoxidase staining of a whole-mount embyro at 24 hours of development.