Western Blot: VSIG2 Antibody (2D8) [NBP2-03376] Analysis of extracts (35ug) from 9 different cell lines by using anti-VSIG2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/Immunofluorescence: VSIG2 Antibody (2D8) [NBP2-03376] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VSIG2.
Immunohistochemistry-Paraffin: VSIG2 Antibody (2D8) [NBP2-03376] - Staining of paraffin-embedded Human prostate tissue using anti-VSIG2 mouse monoclonal antibody.
Flow Cytometry: VSIG2 Antibody (2D8) [NBP2-03376] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-VSIG2 antibody, and then analyzed by flow cytometry.
Western Blot: VSIG2 Antibody (2D8) [NBP2-03376] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSIG2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSIG2.