Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse CCL3/MIP‑1 alpha Monoclonal Antibody (Catalog # MAB4501) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CCL3/MIP‑1 alpha at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CCL3/MIP‑1 alpha was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line stimulated (upper panel) or not (lower panel) with LPS using Rat Anti-Mouse CCL3/MIP‑1 alpha Monoclonal Antibody (Catalog # MAB4501) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of stimulated cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.