Western blot shows lysates of human mesenchymal stem cells either undifferentiated or differentiated into adipocytes. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CTRP3/C1qTNF3/CORS26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7925) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CTRP3/C1qTNF3/CORS26 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CTRP3/C1qTNF3/CORS26 was detected in immersion fixed human mesenchymal stem cells differentiated (upper panel) or not (lower panel) into adipocytes using Sheep Anti-Human CTRP3/C1qTNF3/CORS26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7925) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue. Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.