Western blot shows lysates of P19 mouse embryonal carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Human/Mouse DCBLD2/ESDN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6269) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DCBLD2/ESDN at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
NCI‑H460 human large cell lung carcinoma cell line was stained with Human/Mouse DCBLD2/ESDN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6269, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).