Crossveinless‑2/CV‑2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Human Crossveinless‑2/CV‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1956) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.