Western blot shows human cerebellum tissue lysate and cerebrospinal fluid (CSF). Recombinant Human (rh) Contactin‑2/TAG1 (Catalog # 1714-CN) and conditioned media from BG01V human embryonic stem cells were loaded as positive and negative controls, respectively. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Contactin‑2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1714) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Contactin‑2/TAG1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Contactin‑2/TAG1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human Contactin‑2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1714) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.