Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GPRASP2 Monoclonal Antibody (Catalog # MAB7078) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GPRASP2 at approximately 94 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GPRASP2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human GPRASP2 Monoclonal Antibody (Catalog # MAB7078) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.