Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human AG‑3 Monoclonal Antibody (Catalog # MAB6307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for AG‑3 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
AG‑3 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti‑Human AG‑3 Monoclonal Antibody (Catalog # MAB6307) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of glandular cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.