Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human ONECUT2 Monoclonal Antibody (Catalog # MAB6294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ONECUT2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ONECUT2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human ONECUT2 Monoclonal Antibody (Catalog # MAB6294) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei (punctate). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.