Western blot shows lysates of Hs 294T human melanoma cell line, SK‑Mel‑28 human malignant melanoma cell line, A375 human melanoma cell line, and Bowes human melanoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ST8 alpha ‑2,8‑Sialyltransferase 8A/ST8SIA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6716) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST8 alpha ‑2,8‑Sialyltransferase 8A/ST8SIA1 at approximately 48‑50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ST8 alpha ‑2,8‑Sialyltransferase 8A/ST8SIA1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human ST8 alpha ‑2,8‑Sialyltransferase 8A/ST8SIA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6716) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.