Western blot shows lysates of human testis tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human GAPDH‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6276) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GAPDH‑2 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GAPDH‑2 was detected in immersion fixed paraffin-embedded sections of human testes using Goat Anti-Human GAPDH‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6276) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of sperm cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.