Western blot shows lysates of human prostate tissue and human testis tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ACP6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7766) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ACP6 at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ACP6 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Sheep Anti-Human ACP6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7766) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.