Western blot shows lysates of bEnd.3 mouse endothelioma cell line, T98G human glioblastoma cell line, Neuro‑2A mouse neuroblastoma cell line, and IMR‑32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat TAFA5/FAM19A5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5148) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TAFA5/FAM19A5 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
IMR‑32 human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/Rat TAFA5/FAM19A5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5148, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).