Western blot shows lysates of CTLL‑2 mouse cytotoxic T cell line, C2C12 mouse myoblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Mouse/Rat Caspase‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1456) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Caspase‑12 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.