Western blot shows lysates of IMR‑32 human neuroblastoma cell line and human brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MEGF9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7768) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MEGF9 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MEGF9 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human MEGF9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7768) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.