Western blot shows lysates of human kidney (medulla) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human SorCS2 Antigen Affinity-purified Antibody (Catalog # AF4238) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SorCS2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
SorCS2 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Sheep Anti-Human SorCS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4238) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and their processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.