Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and SK‑Mel‑28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SNAP29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7869) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SNAP29 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SNAP29 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Sheep Anti-Human SNAP29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7869) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi granules in cytoplasm (STX6 staining shown in green, lower panel). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.