Western blot shows lysates of Y‑79 human retinoblastoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human VIAAT/SLC32A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6847) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for VIAAT/SLC32A1 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
VIAAT/SLC32A1 was detected in immersion fixed paraffin-embedded sections of human brain (caudate putamen) using Sheep Anti-Human VIAAT/SLC32A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6847) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.